forodesine hydrochloride Search Results


reactions immucillin h  (MedChemExpress)
93
MedChemExpress reactions immucillin h
SPRINT can be used for compound screens and enzyme-coupled assays. Fluorescent signal of the SPRINT reactions was background subtracted and normalized to 125 nM fluorescein. ( A ) The guanine riboswitch xpt/pbuE *6U regulated transcription in response to 30 different compounds. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent mean value, n = 3. ( B ) The effect of the compounds on transcription from a constitutive promoter was measured. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent one biological replicate. ( C ) SPRINT measured constitutive transcription of Cas13a target as transcription was inhibited at increasing concentrations of rifampicin. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. ( D ) Diagram of an enzyme-coupled assay. The enzyme hPNP converts inosine to hypoxanthine, which is detected by the guanine riboswitch xpt/pbuE *6U and triggers the SPRINT signal. ( E ) The enzyme-coupled assay was used to measure enzymatic activity of hPNP. Concentration of inosine was 1 mM, hPNP was added to an activity of 10 mU/μl, concentration <t>of</t> <t>Immucillin-H</t> was 10 μM. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. p -value was calculated using a two-tailed t -test. ( F ) The enzyme-coupled assay was used to measure concentration-dependent substrate conversion. hPNP was added to an activity of 1 mU/μl. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3.
Reactions Immucillin H, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reactions immucillin h/product/MedChemExpress
Average 93 stars, based on 1 article reviews
reactions immucillin h - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
forodesine hydrochloride 36  (Mundipharma gmbh)
90
Mundipharma gmbh forodesine hydrochloride 36
SPRINT can be used for compound screens and enzyme-coupled assays. Fluorescent signal of the SPRINT reactions was background subtracted and normalized to 125 nM fluorescein. ( A ) The guanine riboswitch xpt/pbuE *6U regulated transcription in response to 30 different compounds. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent mean value, n = 3. ( B ) The effect of the compounds on transcription from a constitutive promoter was measured. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent one biological replicate. ( C ) SPRINT measured constitutive transcription of Cas13a target as transcription was inhibited at increasing concentrations of rifampicin. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. ( D ) Diagram of an enzyme-coupled assay. The enzyme hPNP converts inosine to hypoxanthine, which is detected by the guanine riboswitch xpt/pbuE *6U and triggers the SPRINT signal. ( E ) The enzyme-coupled assay was used to measure enzymatic activity of hPNP. Concentration of inosine was 1 mM, hPNP was added to an activity of 10 mU/μl, concentration <t>of</t> <t>Immucillin-H</t> was 10 μM. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. p -value was calculated using a two-tailed t -test. ( F ) The enzyme-coupled assay was used to measure concentration-dependent substrate conversion. hPNP was added to an activity of 1 mU/μl. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3.
Forodesine Hydrochloride 36, supplied by Mundipharma gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/forodesine hydrochloride 36/product/Mundipharma gmbh
Average 90 stars, based on 1 article reviews
forodesine hydrochloride 36 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
forodesine hydrochloride  (BOC Sciences)
93
BOC Sciences forodesine hydrochloride
SPRINT can be used for compound screens and enzyme-coupled assays. Fluorescent signal of the SPRINT reactions was background subtracted and normalized to 125 nM fluorescein. ( A ) The guanine riboswitch xpt/pbuE *6U regulated transcription in response to 30 different compounds. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent mean value, n = 3. ( B ) The effect of the compounds on transcription from a constitutive promoter was measured. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent one biological replicate. ( C ) SPRINT measured constitutive transcription of Cas13a target as transcription was inhibited at increasing concentrations of rifampicin. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. ( D ) Diagram of an enzyme-coupled assay. The enzyme hPNP converts inosine to hypoxanthine, which is detected by the guanine riboswitch xpt/pbuE *6U and triggers the SPRINT signal. ( E ) The enzyme-coupled assay was used to measure enzymatic activity of hPNP. Concentration of inosine was 1 mM, hPNP was added to an activity of 10 mU/μl, concentration <t>of</t> <t>Immucillin-H</t> was 10 μM. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. p -value was calculated using a two-tailed t -test. ( F ) The enzyme-coupled assay was used to measure concentration-dependent substrate conversion. hPNP was added to an activity of 1 mU/μl. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3.
Forodesine Hydrochloride, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/forodesine hydrochloride/product/BOC Sciences
Average 93 stars, based on 1 article reviews
forodesine hydrochloride - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
oral formulation of forodesine hydrochloride  (BioCryst Inc)
90
BioCryst Inc oral formulation of forodesine hydrochloride
SPRINT can be used for compound screens and enzyme-coupled assays. Fluorescent signal of the SPRINT reactions was background subtracted and normalized to 125 nM fluorescein. ( A ) The guanine riboswitch xpt/pbuE *6U regulated transcription in response to 30 different compounds. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent mean value, n = 3. ( B ) The effect of the compounds on transcription from a constitutive promoter was measured. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent one biological replicate. ( C ) SPRINT measured constitutive transcription of Cas13a target as transcription was inhibited at increasing concentrations of rifampicin. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. ( D ) Diagram of an enzyme-coupled assay. The enzyme hPNP converts inosine to hypoxanthine, which is detected by the guanine riboswitch xpt/pbuE *6U and triggers the SPRINT signal. ( E ) The enzyme-coupled assay was used to measure enzymatic activity of hPNP. Concentration of inosine was 1 mM, hPNP was added to an activity of 10 mU/μl, concentration <t>of</t> <t>Immucillin-H</t> was 10 μM. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. p -value was calculated using a two-tailed t -test. ( F ) The enzyme-coupled assay was used to measure concentration-dependent substrate conversion. hPNP was added to an activity of 1 mU/μl. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3.
Oral Formulation Of Forodesine Hydrochloride, supplied by BioCryst Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oral formulation of forodesine hydrochloride/product/BioCryst Inc
Average 90 stars, based on 1 article reviews
oral formulation of forodesine hydrochloride - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


SPRINT can be used for compound screens and enzyme-coupled assays. Fluorescent signal of the SPRINT reactions was background subtracted and normalized to 125 nM fluorescein. ( A ) The guanine riboswitch xpt/pbuE *6U regulated transcription in response to 30 different compounds. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent mean value, n = 3. ( B ) The effect of the compounds on transcription from a constitutive promoter was measured. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent one biological replicate. ( C ) SPRINT measured constitutive transcription of Cas13a target as transcription was inhibited at increasing concentrations of rifampicin. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. ( D ) Diagram of an enzyme-coupled assay. The enzyme hPNP converts inosine to hypoxanthine, which is detected by the guanine riboswitch xpt/pbuE *6U and triggers the SPRINT signal. ( E ) The enzyme-coupled assay was used to measure enzymatic activity of hPNP. Concentration of inosine was 1 mM, hPNP was added to an activity of 10 mU/μl, concentration of Immucillin-H was 10 μM. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. p -value was calculated using a two-tailed t -test. ( F ) The enzyme-coupled assay was used to measure concentration-dependent substrate conversion. hPNP was added to an activity of 1 mU/μl. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3.

Journal: Nucleic Acids Research

Article Title: SPRINT: a Cas13a-based platform for detection of small molecules

doi: 10.1093/nar/gkaa673

Figure Lengend Snippet: SPRINT can be used for compound screens and enzyme-coupled assays. Fluorescent signal of the SPRINT reactions was background subtracted and normalized to 125 nM fluorescein. ( A ) The guanine riboswitch xpt/pbuE *6U regulated transcription in response to 30 different compounds. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent mean value, n = 3. ( B ) The effect of the compounds on transcription from a constitutive promoter was measured. Signal with solvent only was subtracted from signal with ligand to correct for differences in solvents of ligands. Dots represent one biological replicate. ( C ) SPRINT measured constitutive transcription of Cas13a target as transcription was inhibited at increasing concentrations of rifampicin. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. ( D ) Diagram of an enzyme-coupled assay. The enzyme hPNP converts inosine to hypoxanthine, which is detected by the guanine riboswitch xpt/pbuE *6U and triggers the SPRINT signal. ( E ) The enzyme-coupled assay was used to measure enzymatic activity of hPNP. Concentration of inosine was 1 mM, hPNP was added to an activity of 10 mU/μl, concentration of Immucillin-H was 10 μM. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. p -value was calculated using a two-tailed t -test. ( F ) The enzyme-coupled assay was used to measure concentration-dependent substrate conversion. hPNP was added to an activity of 1 mU/μl. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3.

Article Snippet: Additionally, the following components were added: human purine nucleoside phosphorylase (Sigma-Aldrich, # 540221) was added to 0.01 U/μl, MgHPO 4 was added to 1 mM, in some reactions Immucillin-H (also known as forodesine) (MedChemExpress, #HY-16209) was added to 10 μM.

Techniques: Solvent, Activity Assay, Concentration Assay, Two Tailed Test